Addition of 3′ A overhangs to PCR products

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Addition of 3′ A overhangs to PCR products

2016年2月8日 科技 0

If PCR is performed using a proofreading DNA polymerase, such as Pfu DNA polymerase, the product will have blunt ends. Taq DNA polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning.

Details: http://openwetware.org/wiki/Addition_of_3’_A_overhangs_to_PCR_products