科技

siRNA and shRNA Design Guidelines – Review

June/July 2003 The design of siRNAs and short hairpin siRNAs (shRNAs) remains an empirical process since the molecular mechanisms underlying RNAi are not yet sufficiently understood to allow for the rational design of siRNAs. However, based on the research from various laboratories including our own, InvivoGen has been able to develop siRNA Wizard, an online tool accessible from our homepage, that will help you find the best siRNA sequences on your target gene. The siRNA…

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科技

RNAi in good Cell growing

Cells growing in log phase are the most suitable one for studying gene silencing because once the cells come to be in confluence, cell cycle will be blocked, senescence will start and a group of inhibins will be secreted. All these will affect the physiological status of the cells and in turn affect gene silencing. I am assuming that the protein of interest is not density- or cell-cycle-dependent, otherwise the experimental needs are dictated by…

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