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Addition of 3′ A overhangs to PCR products

If PCR is performed using a proofreading DNA polymerase, such as Pfu DNA polymerase, the product will have blunt ends. Taq DNA polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning. Details: http://openwetware.org/wiki/Addition_of_3’_A_overhangs_to_PCR_products

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pan-neuronal genes

Despite remarkable variation in morphology and function, all neurons possess a set of attributes that allow their assignment to this cell type (Kandel et al., 2000). These features are likely determined by genes, called pan-neuronal genes, uniquely expressed in all (or nearly all) neurons, but not in other cell types. However, in many instances broadly expressed neuronal genes are also expressed in other cell types (Iwasaki et al., 1997; Nonet et al., 1999; Rajaram et…

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